LOCUS       40924_23784       12198 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB727 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12198)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12198)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12198)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1210
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     protein_bind    1220..1344
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1381..1411
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1465..2121
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2466..2768
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2812..2936)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2962..3214
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3248..3264)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3272..3288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3296..3326)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3341..3362)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3398..3650)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3688..4983)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61238.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3688..4983)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5035..5214)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5720..5744)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6265..7053
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7302..7890
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8076..8216)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8560..8754)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8823..9893)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10325..10951)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gctagcatga ctggtggaca
     1201 gcaaatgggt gctgttatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
     1261 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     1321 aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg caccccaggc
     1381 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     1441 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     1501 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     1561 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     1621 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     1681 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     1741 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     1801 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     1861 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     1921 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     1981 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     2041 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     2101 gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag
     2161 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     2221 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
     2281 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     2341 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     2401 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     2461 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     2521 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     2581 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     2641 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     2701 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     2761 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
     2821 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     2881 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgata
     2941 acctagagct cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg
     3001 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat
     3061 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
     3121 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
     3181 ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tggttccgga accaattcgt
     3241 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3301 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3361 taggctgaat taggcgcgcc tatttctatt aattcccgat ctagtaacat agatgacacc
     3421 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     3481 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     3541 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     3601 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc
     3661 gcctcgagtg acttctctga ttgcagacta tttataccaa ccagcaagaa agtgtcgaag
     3721 tatgaagcag aaggcgcagg ctagagcctg gaaaacgaga agatggatgc aaagagactt
     3781 ttctgattta ggaccaaaca acctcaagta aacattgacg agtgtcccat cttttgtgcc
     3841 ggtcagtaat ccggtagcag gatcatacct tgggagacga tggcgcggac atttcacaat
     3901 cccagcaagc tgaggaagcg ataatagaag gttcagaact tgaggaagaa agaagatgag
     3961 gagggtttca ctgaagtgac ccagaatacc aacaactgcc atagtcattc cggcgaagac
     4021 tgtgtaagtg tctccaacaa aaactgaaga aggataccaa ttgtaagcaa gcattgccaa
     4081 ggatgtggcc atcagcggtt gagtaagaaa tatcgagaag gcatgagctt ggtgatactc
     4141 gggatcaaca gatgctccga tttgcataac gttgtgtatc aggatagcag cggcaataac
     4201 aactgtttga ccgatttcaa ggccattcag accagcgtgg atgttaatag agtttgtaca
     4261 aaagacagca agtagcccca tatataactt atatatcctt cctagattaa gcacttccaa
     4321 gccaatataa gcaacaagag gttttggtat aacaatagtt gtatgaccag cataagccat
     4381 caaaagtgga agggtagcaa aagatggcag gacaagtttt actctccaag gcacatcaag
     4441 gacatcatct acaaatccaa gcaaaatcat gaagcaaata gatgctagtg ctgcattata
     4501 ctccacaagc caattagaat cttcagtgaa gttaaaatac tgaaatatta tcgccacgat
     4561 caaaaagaca ataccaacaa caatacccaa tgactctggc actttaatat ctccttgagg
     4621 cgtgcctctt ttgttgatat caaaaccaaa catgttacgt ctcagaacgt atctggcagt
     4681 aacaggaatc attttgagag tgacgaaaaa cccaaccaag ctaagccccg cgttaattag
     4741 aatcgatcgc ttgagttcat tatcaacctt gtagtgaaaa cagagaaggt atagatagag
     4801 cgaacagaga agagtagaga tcacgaagat cactcctaat ttcggcggcg cgagacgaaa
     4861 ttcctcaccg gaagcagaaa ccgtctttcg ggtcatcttc tgctccgatg gcgctgagtt
     4921 gggttccgac gggtccggtt tgttgggaat tgagatcgat gaagctctct tacgagctgc
     4981 catatcttat tgccccccgg gatcagatct ggattgagag tgaatatgag actctaattg
     5041 gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag
     5101 ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta
     5161 gctcattaaa ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag
     5221 ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta
     5281 attctccgct catgatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat
     5341 ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg
     5401 ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc
     5461 tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt
     5521 catccggggt cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca
     5581 gcccttgcgc cctgagtgct tgcggcagcg tgaagctgat ccgtcgagat taatagaaat
     5641 aaattcagcc taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa
     5701 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc
     5761 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
     5821 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
     5881 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
     5941 atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag
     6001 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     6061 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     6121 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     6181 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     6241 cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa
     6301 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     6361 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     6421 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     6481 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     6541 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     6601 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     6661 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     6721 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     6781 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     6841 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     6901 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     6961 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     7021 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag
     7081 ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc
     7141 tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc
     7201 ctacacaaat tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc
     7261 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7321 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7381 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7441 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7501 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7561 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7621 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7681 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7741 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7801 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7861 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7921 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7981 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8041 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     8101 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     8161 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     8221 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8281 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8341 cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg
     8401 gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg
     8461 gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc
     8521 gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt
     8581 ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt
     8641 atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac
     8701 gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag
     8761 agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat
     8821 taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg
     8881 ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc
     8941 ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc
     9001 gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga
     9061 aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg
     9121 ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc
     9181 ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat
     9241 accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg
     9301 tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg
     9361 cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc
     9421 ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg
     9481 taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga
     9541 agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc
     9601 acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa
     9661 tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt
     9721 tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct
     9781 tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca
     9841 tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt
     9901 cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc
     9961 ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt
    10021 cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg
    10081 ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct
    10141 tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc
    10201 cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg
    10261 ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa
    10321 ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc
    10381 cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc
    10441 gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct
    10501 caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc
    10561 catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt
    10621 cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag
    10681 ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct
    10741 tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc
    10801 gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg
    10861 ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc
    10921 ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac
    10981 tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca
    11041 cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag
    11101 cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg
    11161 cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa
    11221 cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg
    11281 gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg
    11341 gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc
    11401 acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg
    11461 gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc
    11521 ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca
    11581 atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc
    11641 tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg
    11701 tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt
    11761 aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg
    11821 gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg
    11881 tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg
    11941 taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga
    12001 aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg
    12061 tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg
    12121 ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat
    12181 ggacgaacgg ataaacct
//
